Showcased in the TV series Gotham where he plays a major antagonist

This lesser known villain got screentime in  the animated series "Beware The Batman"

Psychotic surgeon who obsesses over making people “perfect”.

Mad Hatter has been featured in multiple Batman related video games such as Batman Arkham City and Batman Arkham Asylum

Criminal mastermind who masquerades the  persona of Alice and Wonderland character “Mad Hatter”. He uses mind control to get inside the Dark Knight’s head.

Although this role received backlash from the fans, Whiplash was the main antagonist in Iron Man 2

Russian physicist equipped with armor and energy whips. Vows to deliver vengeance upon the Stark family.

Black Cat has been in multiple Spidey TV Shows and video games but notably Insomniac’s Marvel’s Spider-Man

Featured in 2016’s Suicide Squad as a side character

Uses weaponized boomerang to take down his enemies

Starred in “Gotham” as an antagonist throughout the series

Unhinged Victor Zsasz thinks life is worthless and each of his victims get marked on his body with a scar. 

Major villain in the Spider-Man universe who uses illusions and special effects to trick people and commit crimes

A wealthy and brutal crime lord who dawns a mask enjoys watching others in pain and stops at nothing to turn Gotham into a living hell.

Vulture

Genius engineer creates a specialized suit which allows him to fly and make Spider-Man’s life a living hell

Scarecrow

Jon Crane better known as the Scarecrow uses fear gas on his victims which make them see their greatest nightmares

An experiment gone wrong turns scientist Kirk Langstorm into the creature known as Man-Bat who roams the skies in Gotham with it’s only goal is to wreak havoc

Former lawyer turned criminal. After a horrific accident Harvey utilizes a coin to make insane decisions as a crime lord. If there is one thing Two-Face doesn’t play heads or tails in is destroying Batman once and for all.

One of Batman’s most popular villains Penguin was in numerous Batman TV Shows, Video Games and was portrayed by Danny Devito in Tim Burton's "Batman Returns" 

Criminal mastermind that tries to destroy the Dark Knight with everything he gets a hold of using his wealth.

Joker’s partner in crime was introduced in the highly acclaimed TV series " Batman:The Animated Series". Since then she has been in multiple DC video games, TV shows and recently she just got her own film Birds Of Prey that just hit theaters.

Only a shell of her past life as a therapist, Harley Quinn fell in love with one of her patients known as the Joker and that transformed her into an agile and dangerous villain known for her unpredictability.

Edward Nigma utilizes puzzles and traps for his victims in SAW like fashion while also attempting to accomplish his goal which is to outsmart the Dark Knight.

Childhood friend turned bitter enemy to Bruce Wayne, Hush uses disguises to pose as others and commit crimes.

Featured in Deadpool 2 as a former antagonist

Time Traveling mutant that can create shields and also utlizes telekinesis powers. Although villains are meant to be evil, Cable has a softer side to him as he loved his family greatly before they die and his goal is to kill Russell Collins in Deadpool 2.

Unintentional villain Bizarro has the same abilities as Superman but he lacks his intelligence which causes him to be perceived as a villain because he wants to help but ends up making a bigger mess.

Mandarin is known as the archenemy to Iron Man and he was showcased in Iron Man 3

Arguably the strongest member of the Green Lantern Corps is Sinestro. He soon leaves that and starts the Sinestro Corps in order to destroy Green Lantern's team and dent the universe.

Jean Gray after she became corrupted by the Hellfire Club turns into the vastly power supervillain known as Dark Phoenix. She is argubably the X-Men's greatest challenge as she is a powerful cosmic being known for devouring galaxies

Intelligent android from outer space poised as one of the most dangerous villains in DC comics who wants to reshape the universe

Incredibly gifted scientist who turned into a godlike figure in the Marvel universe. His intentions are to “fix” the world

Featured in Endgame and Infinity War as the main antagonist

Powerful Titan that is unstoppable with his gauntlet that has the power to erase half of the universe with a snap

Darkseid is mostly recognized for being one of the major antagonists in the DC universe and being Superman's archenemy

Godly being in the DC universe who’s only motivation is to take control of the universe and completely removing free will

Mostly known for being one of the deadliest characters in all of comic book history and also known for being the father of Teen Titan’s member Raven

An interdimensional  powerful demonic being who does everything in his power to destroy the universe. He can shapeshift, project energy, drain souls and much more. He's the literal devil of DC Comics.